Journal: Frontiers in Immunology

Article Title: Pan-cancer analysis identifies tRNA modification enzyme CTU2 as a novel tumor biomarker and its role in immune microenvironment

doi: 10.3389/fimmu.2025.1547794

Figure Lengend Snippet: CTU2 knockdown impairs LIHC and KIRC progression in vivo and in vitro . (A) Western blot confirming CTU2 knockdown in huh-7 and 786-O. Control (untransfected wild-type cells), NC (lentiviral empty vector group), Sh-CTU2 (lentivirus-mediated CTU2 knockdown). The lower graph shows the grey value of CTU2 protein levels, normalized to the corresponding GAPDH levels. The experiment was independently repeated three times (** P < 0.01); (B) Colony-formation assay of CTU2 knockdown and control huh-7 and 786-O, representative images (left panel), and the quantitative analysis (right panel, *** P < 0.001); (C) EdU proliferation assay (upper) and the quantitative analysis (lower) of CTU2 knockdown and control cells (* P <0.05, ** P < 0.01); (D) AnnexinV/PI apoptosis assay (upper) and the quantitative analysis (lower) of CTU2 knockdown and control cells (* P <0.05, ** P < 0.01); (E) Matrigel invasion assay of CTU2 knockdown and control 786-O (upper) and huh-7 (lower) cell, representative images (left panel), and quantification analysis (right panel, ** P < 0.01, *** P < 0.001, **** P < 0.0001); (F) Transwell cell migration analysis of CTU2 knockdown and control 786-O (upper) and huh-7 (lower) cell, representative images (left panel), and quantification analysis (right panel); (G) Representative picture of tumors in xenograft nude mice model subcutaneously implanted with CTU2 knockdown and control huh-7 cells; (H) Xenograft tumor weigh (left, n = 5, ** P < 0.01) and xenograft tumor growth curve (right, n = 5, * P < 0.05).

Article Snippet: For apoptosis assays, the Alexa Fluor 488 Annexin V/PI Cell Apoptosis Kit (Vazyme, A211-01, China) was used according to the manufacturer’s instructions.

Techniques: Knockdown, In Vivo, In Vitro, Western Blot, Control, Plasmid Preparation, Colony Assay, Proliferation Assay, Apoptosis Assay, Invasion Assay, Migration

Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Article Title: Early Changes in the Knee of Healer and Non-Healer Mice Following Non-Invasive Mechanical Injury

doi: 10.1002/jor.23413

Figure Lengend Snippet: Characteristics of Antibodies

Article Snippet: Images were captured on an Eclipse E800 microscope (Nikon, Tokyo, Japan) with QImaging Retiga 2000R Fast 1394 camera and MetaMorph software v7.7 (Molecular Devices, Sunnyvale, CA). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Supplier Cat. # Dilution Mouse anti-CIdU Abcam (Cambridge, MA) ab6326 1:100 Rat anti-IdU Abcam (Cambridge, MA) ab8955 1:100 Rabbit anti-COMP Kamiya Biomedical Co. (Seattle, WA) PC-140 1:100 Rabbit anti-aggrecan (G2 domain) Dr. Amanda Fosang (The University of Melbourne, Australia) Gift 1:100 Rat anti-type-II collagen (triple helical domain) Dr. Linda Sandell (Washington University, St. Louis, MO) In house 1:100 Rabbit anti-NITEGE 373 Dr. Amanda Fosang (The University of Melbourne, Australia) Gift 1:100 Alexa Fluor 594 goat anti-rat Abcam (Cambridge, MA) ab150168 1:200 Alexa Fluor 488 goat anti-rabbit Abcam (Cambridge, MA) ab181448 1:200 Alexa Fluor 594 goat anti-rabbit Abcam (Cambridge, MA) ab150092 1:200 Alexa Fluor 488 goat anti-mouse Abcam (Cambridge, MA) ab150113 1:200 Open in a separate window Characteristics of Antibodies In Situ Hybridization Analyses of Aggrecan To determine whether cells synthesized aggrecan after injury, in situ hybridization was performed using 35 S labeled riboprobe as previously described.

Techniques: